Improved MALDI-TOF MS Identification of Mycobacterium tuberculosis by Use of an Enhanced Cell Disruption Protocol

Mycobacterium tuberculosis is the microorganism that causes tuberculosis, a disease affecting millions of people worldwide. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) fast, reliable, and cost-effective method for identification which has been used spp. isolates. However, mycobacteria cell wall rich in lipids, makes it difficult to obtain proteins MALDI-TOF MS analysis. In this study, two preparation protocols were compared: MycoEx, recommended by instrument manufacturer Bruker Daltonics, MycoLyser protocol described herein, MagNA Lyser enhance disruption with ethanol. Cell protein extraction steps performed using H37Rv strain, results compared. The allowed improved Biotyper M. since log(score) values obtained mostly ≥ 1.800 significantly higher than underwent MycoEx processing. reliability was increased as well, considering criteria. view these results, concluded mycobacterial improves method’s efficacy identification.